Basic laboratory calculations for biotechnology/ Lisa A. Seidman

Unit I. Brief review of some basic math techniques Exponents and scientific notation Logarithms Units of measurement Measurements and significant figures Using equations to describe a relationship Unit II. Applications of proportional relationships Ratios and proportions Unit conversions Density Dosages Percents Introduction to concentration problems Preparing aqueous, biological solutions that contain one solute Preparing laboratory solutions that contain more than one solute Dilutions Unit III. Describing relationships with equations and graphs Graphing linear equations Spectrophotometry Graphing exponential equations Unit IV, Introduction to descriptive statistics Descriptive statistics: measures of central tendency Descriptive statistics: measures of dispersion Statistics and graphical methods of describing data Unit V. Biotechnology applications Common calculations relating to animal cell culture techniques Amount and concentration of nucleic acids Calculations relating to common molecular biology techniques The polymerase chain reaction Quantitative PCR Calculations relating to protein purification and analysis